细胞生物学

拉曼光谱是一种无损、无标记的技术。它可以提取化学信息，无需操作基因或使用染色剂或抗体，这样有助于确保结果能够真实地反映细胞的化学组成。

识别细胞

使用雷尼绍拉曼系统可识别并区分细胞，例如：

从正常细胞中识别癌细胞
从分化细胞中识别干细胞
细胞群体中的不同亚态（例如，干细胞和祖细胞）

您可以基于细胞固有的化学指纹信息进行识别，无需已知标记。而且，无需与抗体结合或操作基因。

观察细微的生物细节

通过高空间分辨率共焦拉曼分析，您可以：

原位检查细胞内结构和单个细胞内的生物分子
检查酵母细胞内包含物的化学内含物
检查癌细胞中的脂类内含物，以深入了解脂类代谢

研究细胞群里的单个细胞，并确定细胞到细胞的变化倾向。例如，您可以分析脂类的分布，以及健康细胞和异常细胞里的DNA。

Raman image of human osteosarcoma (bone cancer) cells. [gen]

Raman image of human osteosarcoma (bone cancer) cells.

Raman image of human osteosarcoma (bone cancer) cells, showing the nuclei (green), nucleoli (red), membrane bound organelles (cyan) and the cell body (yellow – thick region, blue – membranous area). Renishaw thanks Dr Frederick Coffman, Rutgers New Jersey Medical School, USA, for providing the cell sample.
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研究活细胞

您可以为您的雷尼绍拉曼系统配备一个细胞培养箱。该箱室可以控制温度、CO₂浓度和湿度，以保持细胞在分析过程中处于正常的生理状态。

与终点实验相比，活细胞数据能够更好地表达任何动态过程。例如，您可以监测细胞对环境变化或药物的响应。这些响应可能表现为新陈代谢或形态的改变或是细胞的死亡（凋亡），所有这些均可以通过拉曼光谱进行检测。

通过3D视图观察细胞内的变化

获取化学信息并生成样品的3D视图，然后利用这些信息和视图验证细胞对物质的摄入。还可以确定细胞及其细胞器的体积。

Images of glioma cells [gen]

Images of glioma cells

a) section from a 3D Raman volume map of a glioma cell. It shows the distribution of the organelles: white/grey (cell body); yellow/orange (nucleus); blue (lipid droplets / membrane-bound organelles). Renishaw thanks Dr Matthew Baker, University of Central Lancashire, UK, for providing the cell sample. b) Raman volume image, showing the nucleus (red) and lipid droplets / membrane-bound organelles, such as Golgi apparatus, endoplasmic reticulum and mitochondria (blue). Renishaw thanks: Dr Alex Berquand, Bruker Nano GmbH, Germany, for collecting and processing the AFM data; Dr Matthew Baker, University of Central Lancashire, UK, for providing the cell sample.
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资料下载：生命科学（细胞）

Application note: Classification of brain glioma tumours using the Renishaw Biological Analyser [en]
Demonstrating discrimination between diseased and healthy brain tissue using the Renishaw RA816 Biological Analyser.

Application note: Classification of brain glioma tumours using the Renishaw Biological Analyser

Demonstrating discrimination between diseased and healthy brain tissue using the Renishaw RA816 Biological Analyser.
[384kB]
Application note: Cell imaging with the inVia confocal Raman microscope [en]
With Renishaw’s inVia confocal Raman microscope you can identify and characterise samples to provide chemical, spatial and structural information on multiple types of molecule, without labelling. It provides rich, detailed, chemical images and highly specific data at high spatial resolution, making it ideal for studying cells.

Application note: Cell imaging with the inVia confocal Raman microscope

With Renishaw’s inVia confocal Raman microscope you can identify and characterise samples to provide chemical, spatial and structural information on multiple types of molecule, without labelling. It provides rich, detailed, chemical images and highly specific data at high spatial resolution, making it ideal for studying cells.
[365kB]
Application note: Surface enhanced Raman spectroscopy (SERS) imaging using the inVia confocal Raman microscope [en]
Raman imaging is a powerful research tool for understanding the molecular composition, structure and distribution of different chemical species. Nano silver/gold colloids and roughened metallic substrates can be used to amplify the intensity of the Raman scattering of adsorbed molecules via SERS. This can increase the sensitivity and/or the specificity of the analysis. SERS imaging can be used to evaluate the efficacy of delivery of nanoparticles (NPs) into cells/animals. SERS measurement of labelled or surface-modified NPs can also be used for biosensing, multiplexing and theranostics.

Application note: Surface enhanced Raman spectroscopy (SERS) imaging using the inVia confocal Raman microscope

Raman imaging is a powerful research tool for understanding the molecular composition, structure and distribution of different chemical species. Nano silver/gold colloids and roughened metallic substrates can be used to amplify the intensity of the Raman scattering of adsorbed molecules via SERS. This can increase the sensitivity and/or the specificity of the analysis. SERS imaging can be used to evaluate the efficacy of delivery of nanoparticles (NPs) into cells/animals. SERS measurement of labelled or surface-modified NPs can also be used for biosensing, multiplexing and theranostics.
[333kB]
应用说明：通过拉曼成像分析木材细胞和组织中的成分和代谢产物
使用inVia™共焦显微拉曼光谱仪分析欧洲赤松木，揭示其结构和化学组分的高分辨率细节。

应用说明：通过拉曼成像分析木材细胞和组织中的成分和代谢产物

使用inVia™共焦显微拉曼光谱仪分析欧洲赤松木，揭示其结构和化学组分的高分辨率细节。
[489kB]
Portrait of a dying cell [en]
The December 2015 issue of 'The Pathologist' featured an article describing how Raman spectroscopy is a non-invasive way of obtaining morphological and chemical information about cells that may lead to better cancer research.

Portrait of a dying cell

The December 2015 issue of 'The Pathologist' featured an article describing how Raman spectroscopy is a non-invasive way of obtaining morphological and chemical information about cells that may lead to better cancer research.
[1.4MB]

您或许对以下文献感兴趣：

Lau et al (2014) Biomedical Spectroscopy and Imaging 3: 237-247
McAughtrie et al (2013) Chem Sci 4: 3566-72
Kim et al (2010) Anal Bioanal Chem 398: 3051-3061

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